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Journal:
Article Title: Generation of a Syngeneic Mouse Model to Study the Effects of Vascular Endothelial Growth Factor in Ovarian Carcinoma
doi:
Figure Lengend Snippet: GFP expression is stable in vivo and provides a sensitive tool to monitor tumor growth and metastasis. Stable expression of enhanced GFP in vivo allows for rapid identification of tumors in both flank and intraperitoneal models. A-F: Flank tumors resected from both sides of the same mouse. The borders between the tumor and normal tissue can be easily observed owing to the distribution of GFP fluorescence. Furthermore, the nonluminous tumor-associated blood vessels are clearly observed against the fluorescence of the GFP-expressing tumors under the fluorescent stereomicroscope. In control tumors from GFP-transfected ID8 cells (A and B), few blood vessels grow into the tumor; whereas in tumors from VEGF/GFP-transfected ID8 cells (C and D), prominent blood vessels growing into the tumor from nearby normal tissue are observed. Tumor from GFP-transfected ID8 cells under light microscope (E) and fluorescence stereomicroscope (F). A large central necrosis area is observed. Note the absence of prominent vessels in comparison with (D). Areas of necrosis were absent in tumors from VEGF/GFP-transfected ID8 cells. G and H: In the intraperitoneal tumor model, microscopic tumor nodules are detected on the spleen by stereomicroscopy. GFP expression allows for accurate detection of tumors. I: Real-time PCR revealed the presence of GFP gene, the genomic tumor marker, in multiple normal tissues of mice bearing VEGF164/GFP flank tumors, whereas in control mice, GFP was only detected in the lung.
Article Snippet: Live Fluorescent Stereo Microscopy The gross morphology of tumors was observed using a
Techniques: Expressing, In Vivo, Fluorescence, Control, Transfection, Light Microscopy, Comparison, Real-time Polymerase Chain Reaction, Marker